Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (81,335)

Singleplex and Multiplex Bead Based Immunoassays (5,686)

ELISA Substrates and Reagents (2,159)

31 – 45 of 86,576 results

Invitrogen™ Human AMPK alpha-1,2 (Phospho) [pT172] ELISA Kit

ELISA

Pricing & Availability

Invitrogen™ Human SMAD1 (Total/Phospho) InstantOne™ ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	4086

Invitrogen™ Human EGF ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	5.3%
Intraassay CV	4.4
Incubation Time	4 hr.
Gene Symbol	HOMG4,URG
Total Assay Time	4 hrs
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Urine 10 μL
Assay Sensitivity	<1 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	3.9 to 250 pg/mL
Marker Gene	EGF
For Use With (Application)	Colorimetric Detection
Test Time	1 hrs 20 mins
Accession Number	P01133
Regulatory Status	RUO
Target	EGF
Kit Contents	Pre-coated 96 well plate,Standard,Standard Dilution Buffer,Biotinylated Detection Antibody,Streptavidin-HRP,HRP Diluent,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	1950
Sample Type	Plasma,Serum,Supernatant,Urine
Label or Dye	HRP

Invitrogen™ IL-10 Human ProcartaPlex™ Simplex Kit

Ideal solution for assessing multiple protein biomarkers in a single sample. Explore our broad portfolio of pre-configurated panels and combinable single analytes.

Pricing & Availability

Invitrogen™ Human IL-21 Uncoated ELISA Kit

ELISA

Pricing & Availability

Invitrogen™ Human tPA ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	6.5%
Intraassay CV	3.6
Gene Symbol	T-PA,TPA
Total Assay Time	2 hrs 10 mins
Sample Volume	Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	6 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	15.6 to 1,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 20 mins
Accession Number	P00750
Regulatory Status	RUO
Target	tPA
Kit Contents	Pre-coated 96 well plate,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Controls,Adhesive Plate Covers
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	5327
Sample Type	Serum,Supernatant
Label or Dye	HRP
Synonym	Tissue-type plasminogen activator

Invitrogen™ Mouse IgG2a Uncoated ELISA Kit with Plates

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Total Assay Time	24 hr 30 min
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 50 μL
Assay Sensitivity	3.90 ng/mL
Storage Requirements	2-8°C
Assay Range	3.90-250 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Regulatory Status	RUO
Target	Mouse IgG2a
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Immunoglobulin G2a

Invitrogen™ Human IL-4 ELISA Kit

ELISA

Pricing & Availability
Specifications

For Use With (Equipment)	Colorimetric Microplate Reader
Gene ID (Entrez)	3565
Interassay CV	4.5%
Intraassay CV	3
Assay Sensitivity	<2 pg/mL
Assay Range	7.8 to 500 pg/mL
For Use With (Application)	Colorimetric

Invitrogen™ Human HIF1A ELISA Kit

ELISA

Pricing & Availability
Specifications

Measurement	Absorbance at 450 nm minus absorbance (at 550 nm)
Protein Family	Transcription factors
Immunoassay Kit Format	Colorimetric ELISA (TMB substrate) with Plates
Host Species	Human
Target Species	Rat,Mouse
Conjugate	HRP
Intraassay CV	<10
Substrate Type	TMB
Total Assay Time	4 hrs
Sample Volume	Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL
Assay Sensitivity	30 pg/mL
Research Discipline	Immunology
For Use With (Application)	Protein Assays and Analysis
Test Time	1 hrs 20 mins
Detection Method	Colorimetric
Target	HIF1A
Specificity	Specific to human HIF-1 A and shows cross-reactivity with mouse and rat HIF-1 A
Kit Contents	Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Measuring Range	450 to 550 nm
For Use With (Equipment)	Colorimetric Microplate Reader
Label or Dye	HRP
Interassay CV	<10%
Gene Symbol	HIF-1-alpha,HIF-1 A,HIF-1 alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
Storage Requirements	2°C to 8°C
Assay Range	81.92 to 20,000 pg/mL
Accession Number	Q16665
Regulatory Status	RUO
Precision	Intra-assay CV <10%; inter-assay CV <10%
Product Type	ELISA
Gene ID (Entrez)	3091
Sample Type	Cell Lysates,Plasma,Serum

Invitrogen™ Human IL-2 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	Il-1 A
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 250 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P60568
Regulatory Status	RUO
Target	IL-2
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3558
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-2

Invitrogen™ Mouse IL-6 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	IL-21
Total Assay Time	24 hrs 30 mins
Sample Volume	Cell Lysate 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P08505
Regulatory Status	RUO
Target	IL-6
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16193
Sample Type	Cell Lysates,Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-6

Invitrogen™ Human IL-10 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	BCGF-1,BCGF1,BSF-1,BSF1,IL-4
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	2 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	2 to 300 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P22301
Regulatory Status	RUO
Target	IL-10
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3586
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-10

Invitrogen™ Human IL-12 p70 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	IFN-g,Ifg
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P29459,P29460
Regulatory Status	RUO
Target	IL-12 p70
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Streptavidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3592, 3593
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interleukin-12, IL12, IL23

Invitrogen™ Human IFN gamma Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Gene Symbol	IL-12p35,Il-12 A,Ll12a,p35,Il-12 b,Il-12p40,Il12p40,p40
Total Assay Time	24 hrs 30 mins
Sample Volume	Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Assay Sensitivity	4 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	4 to 500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P01579
Regulatory Status	RUO
Target	IFN gamma
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Enzyme: Pre-titrated Avidin-HRP,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	3458
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Interferon-gamma, IFN-g, IFNg

Invitrogen™ Mouse TNF alpha Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	AA690181,IL-15 RA,AI503618,IL-15
Total Assay Time	24 hrs 30 mins
Sample Volume	Lavage Fluid 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Tissue Homogenate 100 μL
Assay Sensitivity	8 pg/mL
Storage Requirements	2°C to 8°C
Assay Range	8 to 1,000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hrs 45 mins
Accession Number	P06804
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Colorimetric Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21926
Sample Type	Lavage Fluid,Plasma,Serum,Supernatant,Tissue Homogenates
Label or Dye	HRP
Synonym	Tumor Necrosis Factor alpha, tnf-a,tnf alpha

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